2016年1月16日星期六

Thermophilic Enzyme Isolated From Famous Hot Springs in South Sulawesi, Indonesia

Thermophiles and especially thermophilic enzymes have to date gained a great deal of interest both as analytical tools, and as biocatalysts for application in large scale.

By Muhammad Zakir

Being in the mountains, the atmosphere at Lejja Hot Springs in Soppeng feels much cooler. But do not imagine the water is icy cold because warm water will be found here. In fact, water from the hill that flows in some tributaries before it flowed into the swimming-pool could boil an egg until cooked grains for consumption. The water temperature reached 60 degrees Celsius, plus sulfur content, sulfur content in accordance with the results of the study reached 1.5 percent. Excess water from the hills is believed efficacious in Soppengwhich can cure various skin diseases and bone, such as rheumatism, tension in the muscles of the body, itching, up acne. The view at tourist sites is increasingly perfect baths with views of the spring that flows in between the slopes of the mountain of evidence around him.

Sampling location at Lejja Hot Springs in Soppeng Regency, 
Province of South Sulawesi, Indonesia
In this location, we have isolated a thermophilic bacteria that producing amylase.
Isolate of RSAII-1b at 50oC for48 h

Thermophilic bacteria are extremophiles; they live in the sorts of temperatures that would toast other organisms. Strictly speaking they are not bacteria, but members of a different branch of life known as Archaea.
Thermophilicbacterias(also known as thermostable enzymes and microorganisms) have been topics for much research during the last two decades, but the interest in thermophiles and how their proteins are able to function at elevated temperatures. Microorganisms are, based on their optimal growth temperatures, divided into three main groups, i.e. psychrophiles (below 20°C), mesophiles (moderate temperatures), and thermophiles (high temperatures, above 55°C). Only few eukaryotes are known to grow above this temperature, but some fungi grow in the temperature range 50 – 55°C. Most thermophilic bacteria characterised today grow below the hyperthermophilic boundary (with some exceptions, such as Thermotoga and Aquifex) while hyperthermophilic species are dominated by the Archaea.
Thermophiles and especially thermophilic enzymes have to date gained a great deal of interest both as analytical tools, and as biocatalysts for application in large scale. Utilization of these enzymes is however still today, despite many efforts, often limited by the cost of the enzymes. With an increasing market for the enzymes, leading to production in higher volumes, the cost is however predicted to decrease. Moreover, with a paradigm shift in industry moving from fossils towards renewable resource utilization, the need of microbial catalysts is predicted to increase, and certainly there will be a continued and increased need of thermostable selective biocatalysts in the future.
Story source: http://bit.ly/1lh5LYp


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